Ordering Information
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Why cut your blot into strips? The Surf-Blot can be used for any technique that formerly required cutting the blot into strips. The Surf-Blot eliminates this step by clamping liquid channels onto the surface of a blot. In a typical experiment, antigen is electrophoresed on a flat-topped gel; the gel is transferred to blotting media and the resultant blot is clamped into the Surf-Blot. Antibody solutions are then incubated in the liquid channels. After rinsing, the blot is removed from the Surf-Blot and the blot visualization step is performed on the intact blot. Since there are no narrow strips to line up, the entire screening set can be compared on the intact blot. The Surf-Blot 10 can also be used for "Grid-Blot" screening of monoclonal antibodies.1 This procedure also allows for rapid quantification of antibodies and antigens.
The Surf-Blot is the only blot screening device that has a convex stainless steel backing plate. Competitive designs rely upon the clamping of two pieces of flat plastic together, often leading to leakage between channels.
A study done at the Harvard School of Public Health shows there is no leakage between Surf-Blot channels with incubations up to 12 hours long. Results of this study.
1. Lane, R.D. et al., 1990, Hybridoma, 8, p. 661.
Other references can be found in the Bibliography.
| Catalog Number | Product Description |
Number of Channels | Channel Length | Channel Width | Blot Width | Sample Volume | Price (U.S. $) |
|---|---|---|---|---|---|---|---|
| 5006 | Surf-Blot 6 | 30 | 6.0 cm. | 1.5 mm. | 8.9 cm. | 60-90µl | 220.00 |
| 5007 | Surf-Blot 7 | 30 | 7.0 cm. | 1.5 mm. | 8.9 cm. | 70-105µl | 240.00 | 5009 | Surf-Blot 9 | 30 | 8.9 cm. | 1.5 mm. | 8.9 cm. | 90-135µl | 240.00 |
| 5010 | Surf-Blot 10 | 30 | 10.1 cm. | 1.5 mm. | 8.9 cm. | 100-150µl | 240.00 |
| 5013 | Surf-Blot 13 | 30 | 13.5 cm. | 1.5 mm. | 8.9 cm. | 130-185µl | 260.00 |
| 5055 | Surf-Blot 5.5 | 21 | 5.5 cm. | 2.5 mm. | 9.3 cm. | 100-140µl | 220.00 |
| 5075 | Surf-Blot 7.5 | 21 | 7.5 cm. | 2.5 mm. | 9.3 cm. | 100-200µl | 240.00 |
| 5085 | Surf-Blot 8.5 | 33 | 8.5 cm. | 2.5 mm. | 14.7 cm. | 100-220µl | 240.00 |
| 5105 | Surf-Blot 10.5 | 21 | 10.5 cm. | 2.5 mm. | 9.3 cm. | 100-300µl | 240.00 |
| 5135 | Surf-Blot 13.5 | 33 | 13.5 cm. | 2.5 mm. | 14.7 cm. | 100-350µl | 290.00 |