Take a PubMed online look at some data produced by Idea Scientific equipment.
Put the PMID number in the search window at pubmed.gov Underlined numbers indicate free full text.
"Human Stx-2-Specific Monoclonal Antibodies Prevent Systemic Complications of Escherichia coli O157:H7 Infection", Mukherjee, J., et. al., Infection and Immunity, Vol 70, pp. 612-619 (2002).
Fig 1 of this paper shows monoclonal antibody screening on an intact western blot. (PMID:11796590)
"Role of protein kinase C in light adaptation of molluscan microvillar photoreceptors", Piccoli, G. et al., Journal of Physiology, Vol. 543.2, pp. 481-494 (2002).
Figure 3 of this paper shows polyclonal antibody screening with a Surf-Blot. (PMID: 12205183)
"Inhibition and enhancement of odorant-induced cAMP accumulation in rat olfactory cilia by antibodies directed against different protein subunits". Sinnarajah, S. et. al., FEBS Letters, Vol. 426, pp. 377-380 (1998).
Surf-Blot used for Figure 3A. (PMID: 9600270)
"Merlin, the Neurofibromatosis Type 2 Gene Product, and beta1 Integrin Associate in Isolated and Differentiating Schwann Cells", Obremski, V.J., et. al., Journal of Neurobiology, Vol. 37, pp. 487-501 (1998).
Surf-Blot used for figures 1,2,3,5, and 6. (PMID: 9858254)
"The Grid-Blot: A Procedure for Screening Large Numbers of Monoclonal Antibodies for Specificity to Native and Denatured Proteins", Lane, R.D., et. al., Hybridoma, Vol. 8, pp. 661-669 (1989).
This paper describes the procedure for Grid-Blot screening, which does not require electrophoresis or blotting. (PMID: 2693340)
"Grid-immunoblotting: a fast and simple technique to test multiple allergens with small amounts of antibody for cross reactivity", Reese, G. et. al., Journal of Chromatography B, Vol. 756, pp. 151-156 (2001).
This refinement of the Grid-Blot procedure examines meat allegies in allergic patients. The procedure uses approximately ten times less antibody than ELISA sera screening. (PMID: 11419706)
"Identification of Continuous, Allergenic Regions of the Major Shrimp Allergen Pen a 1 Tropomyosin" Ayuso, R., et. al., International Journal of Allergy and Immunology, Vol. 127, pp. 27-37 (2002).
Forty-six overlapping peptides spanning the entire tropomyosin molecule are tested with serum from shrimp-allergic patients to map the allergenic regions of tropomyosin. (PMID: 11893851)
"Rapid High Resolution Western Blotting: From Gel to Image in a Single Day", Klapper, A. et. al., Biotechniques, Vol. 12, pp.650-654 (1992).
The 30 minute GENIE transfer step helps shorten the entire Western procedure. (PMID: 1515130)
"Identification of a novel nucleotide-sensitive microtubule-binding protein in HeLa cells", Rickard, J.E. and Kreis, T.E., Journal of Cell Biology, Vol. 110, pp 1623-1633 (1990).
Figures 1, 2 and 3 show that a 170kD protein can be blotted in 30 minutes with a GENIE. (PMID: 1970824)
"The P190, P210, and P230 Forms of the BCR/ABL Oncogene Induce a Similar Chronic Myeloid Leukemia-like Syndrome in Mice but Have Different Lymphoid Leukemogenic Activity", Li, S., et. al., J. Exp. Med., Vol. 189, pp. 1399-1412 (1999).
This paper from laboratories at the Harvard Center for Blood Research, Dana-Farber Cancer Institute and the Whitehead Institute shows the high sensitivity and low background of electroblotting with the Genie. Dr. Van Etten reported that he can detect one-twentieth of a gene copy per diploid genome. (PMID:10224280)
"Extension of Life-Span by Introduction of Telomerase into Normal Human Cells", Bodnar, A. G. et. al., Science, Vol. 279, pp. 349-352 (1998).
This paper was the most highly referenced Southern blot paper of 1998. (PMID: 9454332)
"Higher Order Chromatin Structures in Maize and Arabidopsis", Paul, A. and Ferl, R.J., The Plant Cell, Vol.10, pp. 1349-1359 (1998).
This paper demonstrates the ability to electroblot large, intact, DNA from a CHEF gel without having to process the gel before blotting. (PMID: 9707534)
"Generation of a Zebrafish P1 Artificial Chromosome Library", Amemiya, C.T. and Zon, L.I., Genomics, Vol. 58, pp. 211-213 (1999) and "Generation of a P1 artificial chromosome library of the Southern pufferfish", Amemiya, C.T., et. al., Gene, Vol. 272, pp. 283-289, (2001).
These papers are from laboratories where authentication of library clones requires an extensive number of Southern blots. (PMID: 10366454 and 11470535)
"An Improved Rapid Northern Protocol", Kevil, K.G. et. al., Bioc. and Biop. Res. Comm., Vol. 238, pp. 277-279 (1997).
GENIE transfer of RNA takes 1.5 hours. This procedure does not use formaldehyde or glyoxal. Background is so low that the blocking step is eliminated. (PMID: 9299493)
"Response of rat muscle to acute resistance exercise defined by transcriptional and translational profiling". Chen, Y. et. al., Journal of Physiology, Vol. 545, pp. 27-41 (2002).
Figure 1 shows the distribution of mRNA sizes in polysomes using formaldehyde-agarose gels tansferred to nylon with the GENIE (publication says nitrocellulose membrane, that is incorrect). (PMID: 12433947)
"Adaptations of skeletal muscle to exercise: rapid increase in the transcriptional coactivator PGC-1". Barr, K. et. al. The FASEB Journal, Vol. 16, pp. 1879-1886 (2002).
Westerns to nitrocellulose and formaldehyde agarose Northerns to nylon. (PMID: 12468452)
"Roles of RNase E, Rnase II and PNPase in the degradation of the rps transcripts of Escherichia coli: stabilizing function of RNase II and evidence for efficient degradation in an ams pnp rnb mutant", Hajnsdorf, E., et. al., The EMBO Journal, Vol. 13, pp. 3368-3377 (1994). (PMID: 7519147)
"Comparative Structural Analysis of Nuclear RNse P RNAs from Yeast" Tranguch, A.J. and Engelke, D.R., Journal of Biological Chemistry, Vol. 268, pp. 14045-14053 (1993). (PMID: 8314772)
6% polyacrylamide RNA gels electrotransferred with the GENIE.
I was amazed at the results. Perfect transfer, no background and a great signal. It is incredibly easy to set up and will save my lab hundreds of man hours a year. Congrats on an excellent product.
I have tried quite a number of electrophoretic transfer units over the past 15 years, including tank and semi-dry styles. Although all work to one degree or another, my hands-down favorite unit is the Genie blotter from Idea Scientific