PubMed data produced by Idea Scientific equipment

Take a PubMed online look at some data produced by Idea Scientific equipment.
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GENIE western blots of high molecular weight proteins:

"Aging, but not yet senescent, rats exhibit reduced muscle quality and sarcoplasmic reticulum function" Russ, D.W.,, Acta Physiol, Vol. 201, pp 391-403 (2011). Figure 3 shows a nice Genie blot of 565 kD ryanodyne receptor. The author reports adding 0.01% SDS to the blotting buffer, and blotting overnight at 15 volts. (PMID: 20874807)

"Myotendinous Junction Defects and Reduced Force Transmission in Mice that Lack alpha Integrin and Utrophin" Welser, J.V., et. al. Am. J. Pathol., Vol. 175, pp1545-1554 (2009). Figure 4 shows sharp-banded Genie western blot of 427 kD Dystrophin. (PMID: 19729483)

"Occluden oligomeric assembly at tight junctions of the blood-brain barrier is disrupted by peripheral inflammatory hyperalgesia" McCaffrey, G. et. al., J. Neurochem., Vol. 106, pp. 2395-2409 (2008). Figure 5 shows the sharpness of Genie blots can resolve 220 kD zonula occludens-1 isoforms. (PMID: 18647175)

"Multiple Mechanisms Contribute to Inhibit Transcription in Response to DNA Damage" Heine, G.F. , et. al., J. Biol. Chem., Vol. 283, pp 9555-9561 (2008). Figures 1, 4, 5 and 6 show nice resolution of 210 kD Rpb1 and phosphorylated Rpb1. (PMID: 18281289)

"Age-Related Dystrophin-Glycoprotein Complex Structure and Function in the Rat Extensor Digitorum Longus and Soleus Muscle" Rice, K.M. et. al., J. of Gerontology, Vol.61A, pp.1119-1129 (2006). Figure 5 shows quantitative detection of dystroglycans (250kD) on Genie western blots. (PMID: 17167152)

"Tyrosine kinase inhibitors impair fibroblast growth factor signaling in coronary endothelial cells" Hawker, J.R. Jr. and Granger H.J Am.J. Physiol. Vol 266, pp H107-H120 (1994). Figures 5 and 6 show Genie blots are sharp enough to resolve doublets 2 kD apart on blots of proteins up to 240 kD. (PMID: 8304491)

Using the Surf-Blot for antibody screening on Western blots

"Autoantibody research using the Surf-Blot System" (2013) Review.

"Immunogenicity of mycobacterial vesicles in humans: identification of a new tuberculosis antibody biomarker" Ziegenbalg, A. Tuberculosis, Vol. 93, 448-455 (2013) Surf-Blot data in figure 4 (PMID 23562367)

"Optimal Long-Term Humoral Responses to Replication-Defective Herpes Simplex Virus Require CD21/CD35 Complement Receptor Expression on Stromal Cells", Brockman, Mark A., et. al., J. Virol, Vol. 80, pp. 7111-7117 (2006). Figure 3 of this paper shows testing of mouse sera screening on a western blot using a Surf-Blot. (PMID: 16809316)

"Human Stx-2-Specific Monoclonal Antibodies Prevent Systemic Complications of Escherichia coli O157:H7 Infection", Mukherjee, J., et. al., Infection and Immunity, Vol 70, pp. 612-619 (2002). Fig 1 of this paper shows monoclonal antibody screening on an intact western blot. (PMID:11796590)

"Role of protein kinase C in light adaptation of molluscan microvillar photoreceptors", Piccoli, G. et al., Journal of Physiology, Vol. 543.2, pp. 481-494 (2002). Figure 3 of this paper shows polyclonal antibody screening with a Surf-Blot. (PMID: 12205183)

"Inhibition and enhancement of odorant-induced cAMP accumulation in rat olfactory cilia by antibodies directed against different protein subunits". Sinnarajah, S. et. al., FEBS Letters, Vol. 426, pp. 377-380 (1998). Surf-Blot used for Figure 3A. (PMID: 9600270)

"Merlin, the Neurofibromatosis Type 2 Gene Product, and beta1 Integrin Associate in Isolated and Differentiating Schwann Cells", Obremski, V.J., et. al., Journal of Neurobiology, Vol. 37, pp. 487-501 (1998). Surf-Blot used for figures 1,2,3,5, and 6. (PMID: 9858254)

Using the Surf-Blot for Grid-Blot screening

"The Grid-Blot: A Procedure for Screening Large Numbers of Monoclonal Antibodies for Specificity to Native and Denatured Proteins", Lane, R.D., et. al., Hybridoma, Vol. 8, pp. 661-669 (1989). This paper describes the procedure for Grid-Blot screening, which does not require electrophoresis or blotting. (PMID: 2693340)

"Grid-immunoblotting: a fast and simple technique to test multiple allergens with small amounts of antibody for cross reactivity", Reese, G. et. al., Journal of Chromatography B, Vol. 756, pp. 151-156 (2001). This refinement of the Grid-Blot procedure examines meat allegies in allergic patients. The procedure uses approximately ten times less antibody than ELISA sera screening. (PMID: 11419706)

"Identification of Continuous, Allergenic Regions of the Major Shrimp Allergen Pen a 1 Tropomyosin" Ayuso, R., et. al., International Journal of Allergy and Immunology, Vol. 127, pp. 27-37 (2002). Forty-six overlapping peptides spanning the entire tropomyosin molecule are tested with serum from shrimp-allergic patients to map the allergenic regions of tropomyosin. (PMID: 11893851)

Western Blotting papers using GENIE (from hundreds)

"Rapid High Resolution Western Blotting: From Gel to Image in a Single Day", Klapper, A. et. al., Biotechniques, Vol. 12, pp.650-654 (1992). The 30 minute GENIE transfer step helps shorten the entire Western procedure. (PMID: 1515130)

"Identification of a novel nucleotide-sensitive microtubule-binding protein in HeLa cells", Rickard, J.E. and Kreis, T.E., Journal of Cell Biology, Vol. 110, pp 1623-1633 (1990). Figures 1, 2 and 3 show that a 170kD protein can be blotted in 30 minutes with a GENIE. (PMID: 1970824)

Papers using the GENIE for rapid Southern blots

"The P190, P210, and P230 Forms of the BCR/ABL Oncogene Induce a Similar Chronic Myeloid Leukemia-like Syndrome in Mice but Have Different Lymphoid Leukemogenic Activity", Li, S., et. al., J. Exp. Med., Vol. 189, pp. 1399-1412 (1999). This paper from laboratories at the Harvard Center for Blood Research, Dana-Farber Cancer Institute and the Whitehead Institute shows the high sensitivity and low background of electroblotting with the Genie. Dr. Van Etten reported that he can detect one-twentieth of a gene copy per diploid genome. (PMID:10224280)

"Extension of Life-Span by Introduction of Telomerase into Normal Human Cells", Bodnar, A. G. et. al., Science, Vol. 279, pp. 349-352 (1998). This paper was the most highly referenced Southern blot paper of 1998. (PMID: 9454332)

"Higher Order Chromatin Structures in Maize and Arabidopsis", Paul, A. and Ferl, R.J., The Plant Cell, Vol.10, pp. 1349-1359 (1998). This paper demonstrates the ability to electroblot large, intact, DNA from a CHEF gel without having to process the gel before blotting. (PMID: 9707534)

"Generation of a Zebrafish P1 Artificial Chromosome Library", Amemiya, C.T. and Zon, L.I., Genomics, Vol. 58, pp. 211-213 (1999) and "Generation of a P1 artificial chromosome library of the Southern pufferfish", Amemiya, C.T., et. al., Gene, Vol. 272, pp. 283-289, (2001). These papers are from laboratories where authentication of library clones requires an extensive number of Southern blots. (PMID: 10366454 and 11470535)

Publications using the GENIE for Northern blots

"An Improved Rapid Northern Protocol", Kevil, K.G. et. al., Bioc. and Biop. Res. Comm., Vol. 238, pp. 277-279 (1997). GENIE transfer of RNA takes 1.5 hours. This procedure does not use formaldehyde or glyoxal. Background is so low that the blocking step is eliminated. (PMID: 9299493)

"Response of rat muscle to acute resistance exercise defined by transcriptional and translational profiling". Chen, Y. et. al., Journal of Physiology, Vol. 545, pp. 27-41 (2002). Figure 1 shows the distribution of mRNA sizes in polysomes using formaldehyde-agarose gels tansferred to nylon with the GENIE (publication says nitrocellulose membrane, that is incorrect). (PMID: 12433947)

"Adaptations of skeletal muscle to exercise: rapid increase in the transcriptional coactivator PGC-1". Barr, K. et. al. The FASEB Journal, Vol. 16, pp. 1879-1886 (2002). Westerns to nitrocellulose and formaldehyde agarose Northerns to nylon. (PMID: 12468452)

"Roles of RNase E, Rnase II and PNPase in the degradation of the rps transcripts of Escherichia coli: stabilizing function of RNase II and evidence for efficient degradation in an ams pnp rnb mutant", Hajnsdorf, E., et. al., The EMBO Journal, Vol. 13, pp. 3368-3377 (1994). (PMID: 7519147)

"Comparative Structural Analysis of Nuclear RNse P RNAs from Yeast" Tranguch, A.J. and Engelke, D.R., Journal of Biological Chemistry, Vol. 268, pp. 14045-14053 (1993). (PMID: 8314772) 6% polyacrylamide RNA gels electrotransferred with the GENIE.

What they say about our GENIE blotter:

I was amazed at the results. Perfect transfer, no background and a great signal. It is incredibly easy to set up and will save my lab hundreds of man hours a year. Congrats on an excellent product.

Mark Farman, Associate Profesor, University of Kentucky

I have tried quite a number of electrophoretic transfer units over the past 15 years, including tank and semi-dry styles. Although all work to one degree or another, my hands-down favorite unit is the Genie blotter from Idea Scientific

Michael Campa, Ph.D., Duke University Medical Center ( professional reviews)