genie® electrophoretic transfer

dream blot in minutes

view this video to see how easy it is to use our genie

the genie is the proven electrophoretic transfer apparatus incorporating plate electrodes for the ultimate in field uniformity. its design allows for a very close positioning of the electrodes which, when coupled with a low voltage power supply, creates a high field in volts/cm. the result is rapid and quantitative electrophoretic transfer. with the genie, it is possible to obtain superb western blots in 30 minutes at 24 volts¹,²,⁷ size is not an issue, from micro rna4 to 40 million dalton mucin proteins,⁸. genie can blot any molecule. and, there is a genie sized for any experiment, from blotting a single mini gel to blotting 30 mini gels at once. a 1.5 hour northern blots northern blot procedure was designed for the genie³. and one-hour southern blots are possible without the messy soaking of gels in acid⁵,⁶. million bp dna from chef gels can be blotted as an intact molecule⁹. scientists trust the genie for the most valuable samples. see testimonials.

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western blot	southern blot

click for larger view	agarose chef gel blotted one hour at 12 volts using an Idea Scientific ROYAL GENIE. Blot was soaked in a 0.4 N NaOH ten minutes and probed. Data courtesy of Dr. Robert Haire, All Childrens Hospital, St Petersburg, Florida

BLOTTING LARGE PROTEINS? NO PROBLEM!!!

Semi-dry blotters and dry blotters trap electrode gasses in bubbles that disrupt the field. That is why the transfer of high molecular weight proteins is so poor with dry blotters. The GENIE is loaded horizontally but blotting is in the vertical position, so bubbles rise away from the blotting area. Large proteins like the 565 kD ryanodyne receptor ¹⁰ and 427 kD Dystrophin ¹¹ are blotted well. And the sharp banding of the GENIE allows resolution of 220 kD zonula occulens-1 isoforms ¹² and 210 kD Rpb1 from phosphorylated Rpb1¹³

References

The preceding reference was originally published in BioTechniques 12:5,650-653. Appearing with permission from Eaton Publishing. It is available is an Acrobat PDF file. Use the Free Adobe Acrobat Reader to view it. Please download the latest version for your particular computing platform.

1. Rapid High Resolution Western Blotting: From Gel to Image in a Single Day, Klapper, A., et al., Biotechniques, 12, (1992), pp. 651-654

2. Rickard, J.E., and Kreis, T.E., J. Cell Biology, 110, (1990), pp. 1623-1633.

3. An Improved Rapid Northern Protocol, Kevil, C.G., et. al., B.B.R.C., 238, (1997), pp. 277-279.

4. Bender, W. Genes Dev 22 (2008) pp 14-19. (PMID 18172161)

5. Li, S., et.al. J. Exp. Med., 189, (1999) pp. 1399-1412.

6. Amemiya, C.T. and Zon, L.I., Genomics 58, (1999) pp 211-213.

7. EbolavriusVP35 suppresses IFN production from conventional but not plasmacytiod dendritic cells. Leung, L.W> et. al. Immunol Cell Biol 89 (2011) pp. 792-802 (PMID 21263462).

8. Dr. A. Livraghi-Butrico, personal communication.

9. Paul, A. et al. The Plant Cell, 10, (1998) pp 1349-1359.

10. Russ, D.W., et.al., Acta Physiol, Vol. 201, pp 391-403 (2011) Figure 3. (PMID 20874807)

11. Welser, J.V., et. al. Am. J. Pathol., Vol. 175, pp1545-1554 (2009) Figure 4. (PMID 19729483)

12. McCaffrey, G. et. al., J. Neurochem., Vol. 106, pp. 2395-2409 (2008) Figure 5. (PMID 18647175)

13. Heine, G.F. , et. al., J. Biol. Chem., Vol. 283, pp 9555-9561 (2008). Figures 1, 4, 5 and 6. (PMID 18281289)

Other references can be found in the Bibliography.

What they say about our GENIE blotter:

I was amazed at the results. Perfect transfer, no background and a great signal. It is incredibly easy to set up and will save my lab hundreds of man hours a year. Congrats on an excellent product.

Mark Farman, Associate Profesor, University of Kentucky

I have tried quite a number of electrophoretic transfer units over the past 15 years, including tank and semi-dry styles. Although all work to one degree or another, my hands-down favorite unit is the Genie blotter from Idea Scientific

Michael Campa, Ph.D., Duke University Medical Center (biocompare.com professional reviews)

A study done by the Department of Microbiology-Immunology, Northwestern University shows the advantages of Electroblotting. Details about the study and results can be seen at this link.

ORDERING INFORMATION

The GENIE is offered in five sizes. We recommend apparatus with a platinized titanium anode for all but the most routine western blots. Platinized titanium anodes delivered after October 1, 1997 have a manufacturing date engraved on them, a thicker coating than earlier coated anodes, and have a five year warranty. Blotters include apparatus, anode (+), cathode (-), pads, screens and instructions. Cables are not included with apparatus, but are permanently attached to all power supplies except #4019. Order power supplies and cables below.

GENIE POWER SUPPLIES

These are perfect power supplies for GENIE blotters. They are designed to produce specific voltages, giving greater reproducibility than laboratory power supplies. All power supplies have an input AC cable, and all but #4019 have permanently attached output cables that attach to the Genie blotter.

CONNECTING CABLES

FAQ's

Scientist: Can I reuse my GENIE blotting buffer?

Idea Scientific: We do not recommend reusing the buffer, but many scientists do. Scientists know the importance of individual experiments and decide whether to reuse. Buffer in Premium model blotters remains clear. Here are excerpts from letters from Dr. Leung. His work shows how Ebola virus suppresses Interferon production.

Scientist: Since we used the economy blotters, there was a discernible discoloration of the transfer buffer due to corrosion of the anode. Upon reuse, it tended to stain the membranes, so I typically discarded the buffer after one use. Since the Genie system uses 1/3 to 1/2 the amount in a conventional tank system, we never felt a strong compulsion to reuse the buffer.

Scientist: I used the (new Premium) mini blotter this week, and even though I expected the transfer to work well, the performance of the platinized titanium anode surprised me. In my previous labs, we had used the economy blotter with the alloy anodes, and even though we kept the voltage at 12V for transfers, we always had some corrosion at the anode and some brown discoloration of the transfer buffer. But not with my new blotter! I was able to save the transfer buffer, and will reuse it again today. Plus, I ran the transfer in 30 mins at 24V, getting me out of the lab faster. Thanks!

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